An open library of human kinase domain constructs for automated bacterial expression

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Steven K. Albanese*, Daniel L. Parton*, Mehtap Isik**, Lucelenie Rodríguez-Laureano**, Sonya M. Hanson,  Julie M. Behr, Scott Gradia, Chris Jeans, Nicholas M. Levinson, Markus A. Seeliger, and John D. Chodera.
* co-first author; ** co-second author
Biochemistry 57:4675, 2018. [DOI] [PDF] [bioRxiv] [GitHub]
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Human kinase catalytic domains---the therapeutic target of selective kinase inhibitors used in the treatment of cancer and other diseases---are notoriously difficult and expensive to express in insect or human cells. Here, we utilize the phosphatase co-expression technology developed by Markus Seeliger (now at Stony Brook) to develop a library of human kinase catalytic domains for facile and inexpensive expression in bacteria.